Simultaneous Determination of Candesartan and Hydrochlorothiazide in Human Plasma by HPLC-MS/MS

Introduction. Combined drugs have the greatest efficacy and safety in arterial hypertension treatment. The combination of candesartan hydrochlorothiazide (AT1-receptor antagonist a thiazide diuretic, respectively) provides high efficiency antihypertensive therapy, therefore it is widely used medical practice. Developing method for simultaneous determination hydrochlorithiazide human blood plasma necessary performing analytical part pharmacokinetic studies bioequivalence multicomponent drugs. Aim. aim this study to develop quantitative by high-performance liquid chromatography – tandem mass spectrometry (HPLC-MS/MS) further studies. Materials methods . Determination HPLC-MS/MS. samples were processed acetonitrile protein precipitation. Internal standard: mixed solution valsartan indapamide. Mobile phase: 0.1 % formic acid water (eluent A), B). Column: Phenomenex Luna Phenyl-Hexyl, 50x4.6 mm, 5 μm. Analytical range: 2.00– 300.00 ng/mL candesartan, 2.00–200.00 plasma. Ionization source: electrospray ionization. Detection conditions: 441.10 → 192.00 m/z, 263.15 m/z (candesartan), 295.85 269.00 (hydrochlorothiazide), 436.00 207.05 (valsartan), 363.85 132.10, 189.00 (indapamide). Results discussion This was validated selectivity, matrix effect, calibration curve, accuracy, precision, spike recovery, lower limit quantification, carry-over effect stability. developed meets requirements conducting medicinal products within framework Eurasian Economic Union. Conclusion. range 2.00–300.00 applied BE hydrochlorothiazide.